Developed lectin-modified nanoporous gold monolith for separation and extraction of glycoproteins. Thermogravimetric analysis used to determine the surface coverage on the monoliths. UV detection for in situ determination of tryptophan and tyrosine molar extinction coefficient calculations lab report pdf surface coverage on the nanoporous gold monoliths. Con A-modified nanoporous gold shows selectivity for capture of ovalbumin.
Bound ovalbumin eluted using the competitive ligand α-methyl mannopyranoside. Au monoliths were prepared followed by activation of the terminal carboxyl groups to create amine reactive esters that were utilized in the immobilization of Con A. LA and Con A on np-Au monoliths which were found to be 1. An in situ solution depletion method was developed that enabled surface coverage characterization without damaging the substrate and suggesting the possibility of regeneration. Using this method, the surface coverages of LA and Con A were found to be 0. Extraction of OVA from a 1:3 mole ratio mixture with BSA was demonstrated by the greater amount of depletion of OVA concentration during the circulation with the developed substrate.
A significant amount of captured OVA was eluted using α-methyl mannopyranoside as a competitive ligand. This work is motivated by the need to develop new materials for chromatographic separation and extraction substrates for use in preparative and analytical procedures in glycomics. Check if you have access through your login credentials or your institution. Comprehensive Medicinal Chemistry III – 6. Screen reader users, click the load entire article button to bypass dynamically loaded article content. Please note that Internet Explorer version 8.